Phyllanthus urinaria
Herbs of the genus Phyllanthus in the treatment of chronic hepatitis B: observations with three preparations from different geographic sites.
Henan Institute of Medical Sciences, Henan Medical University, People's Republic of China.
It has been suggested that herbs of the Phyllanthus family may have antiviral activity. We therefore tested the effects of three different Phyllanthus extracts on the serologic status of 123 patients with chronic hepatitis B.
Eleven patients received an extract of Phyllanthus amarus (L) provided by S.P. Thyagarajan, Madras, India.
Forty-two patients received Phyllanthus niruri (L), gathered from Hainan Province in China, and
35 patients received an extract of Phyllanthus urinaria (L), which had been gathered in Henan Province.
Thirty-five control patients received no herbal therapy.
The patients receiving Phyllanthus urinaria (L) were both more likely to lose detectable hepatitis B e-antigen from their serum and more likely to seroconvert hepatitis B e-antibody status from negative to positive
than were patients given either of the other two preparations. No patient changed status with respect to hepatitis B s-antigen.
Researchers in China, India and Great Britain confirm that phyllanthus has significant hepatoprotective properties.* In rodent studies, phyllanthus was found to protect the liver from alcohol and chemical toxins, and to protect chromosomes from damage induced by chemical toxins or radiation.* Phyllanthus may also support aspects of the immune system.* Brazilian researchers showed in 1990 that tea made from phyllanthus increased sodium and creatine excretion.* A 1999
in vitro clinical study demonstrated the inhibition of calcium oxalate crystal formation, and a 2002 in vivo study confirmed the inhibition of the growth of the matrix calculus.* Active ingredients in phyllanthus include the lignans phyllanthine, phyllanthenol, phyllochrysine, phyltetralin, and hypophyllanthine; the bioflavonoids quercetin, quercetol,quercitrin, rutin; and alkaloids, glycosides, saponins, and catechins.
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Phyllanthus urinaria Increases Apoptosis and Reduces Telomerase Activity in Human Nasopharyngeal Carcinoma Cells Address of Corresponding Author Forsch Komplementmed 2009;16:34-40 (DOI: 10.1159/000194154)
Background: This study was designed to obtain the chemical fingerprint and to investigate the effect of Phyllanthus urinaria on telomerase activity and apoptotic pathways in the human nasopharyngeal carcinoma cell line (NPC-BM1). Materials and Methods: The polyphenol compounds in P. urinaria were investigated by HPLC/MS. Cell viability with the treatment of P. urinaria, gallic acid, ellagic acid, quercetin and cisplatin was detected by MTT assay. TUNEL assay, DNA fragmentation analysis and caspase3 activity were used to confirm apoptotic changes. Telomerase activity was determined using the TRAP assay. RNA isolation and RT-PCR were used to analyze the related genes expression. All experiments on treatments with P. urinaria from 0-3 mg/ml were carried out for 24 h. Results: 5 major compounds including gallic acid, brevifolin carboxylic acid, corilagin, phyllanthusiin C and ellagic acid were identified as a plant fingerprint by HPLC/MS. With the MTT assay, we demonstrated that P. urinaria, gallic acid and ellagic acid reduce cell viability. The apoptosis features showed DNA fragmentation and increased caspase-3 activity associated with the down-regulation of Bcl-2, but not of Bax, p53, and PCNA (proliferating cell nuclear antigen) in P. urinaria-treated NPC-BM1 cells. Furthermore, treatment of NPC-BM1 cells led to an inhibition of hTERT (human telomerase reverse transcriptase), hTP1 (human telomerase-associated protein 1) and c-myc mRNA expression and to decreased telomerase activity. Conclusion: This study suggests that P. urinaria induces the death of NPC-BM1 cells in vitro through the induction of apoptosis and inhibited telomerase activity. Copyright © 2009 S. Karger AG, Basel Dr. Sheng-Teng Huang, Department of Chinese Medicine, Chang Gung Memorial Hospital - Kaohsiung Medical Center, Chang Gung Institute of Technology, Taiwan, Republic of China, Tel. +886 7 7317123 ext. 2334, Fax 2335, [email protected] Published_online: February 6, 2009 |
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A COMPARATIVE STUDY OF COMPOUND AND INTERFERON IN THE TREATMENT OF CHRONIC VIRAL HEPATITIS B Wang Xin-hua, Li Chang-qing, Guo Xing-bo and Fu Lin-chun Tropical Medicine Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510407, People’s Republic of China Abstract. groups. Thirty patients were treated with in the treatment group, another 25 patients were treated with domestic recombinant human interferon alpha-1b (IFN- treatment group was 83.3%, showing no significant difference from the control (p>0.05). The normalization rates of ALT, A/G and SB in the treatment group were 73.3%, 80.0% and 78.2% respectively, which were significantly higher than that in the control (p<0.05). The negative conversion rates of HBeAg and HBV-DNA in the treatment group were 42.3% and 47.8%, showing no significant difference from the control (p>0.05). It is indicated that PA Co has remarkable effect for chronic viral hepatitis B in recovery of liver function and inhibition of the replication of HBV.
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