Working with pupae is very difficult!!! It's ok to dissect the tissue from larval or adult males. But how come it's so difficult to do it with pupae!!
I asked my friend how she did it when she was doing her PhD. Her answer made me crazy coz she said "I just sat there and did it over and over until I got it". Oh dear, I probably havent done it over and over yet. Ok I'll try it again and again. Hope I dont give up too soon.